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dentiometric scanner  (Bio-Rad)


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    Bio-Rad dentiometric scanner
    HBx modulated expressions of target proteins in transiently transfected and HBV producing malignant hepatocytes. (A) Western blot confirmed proteins PTEN, p27 and MAP3K (Raf 1) – targets of miR-21, miR-222 and miR-145 respectively were increased accordingly in HBx transfected HepG2 cells. Cells were transfected with 1 μg and 2 μg of HBx plasmid respectively or pCXN2 as a control plasmid. (B) Expressions of PTEN, p27 and MAP3K (Raf 1) proteins in 1.3 fold HBV transfected HepG2 cells. HepG2 cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 vector as a control. (C) Western blot exhibited differential expression of target proteins PTEN, p27 and MAP3K (Raf 1) in constitutively HBV producing HepG2.2.15 and control HepG2 cell line. Cells were collected for protein analysis 48 h after each transfection. β actin was taken as endogenous control. Protein bands were quantified using <t>dentiometric</t> scanner (Bio-Rad). Below are the graphical representation of intensity ratio between target proteins (PTEN, p27 and MAP3K (Raf 1)) and β actin in each lane.
    Dentiometric Scanner, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dentiometric scanner/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    dentiometric scanner - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Tumor suppressor micro RNA miR-145 and onco micro RNAs miR-21 and miR-222 expressions are differentially modulated by Hepatitis B virus X protein in malignant hepatocytes"

    Article Title: Tumor suppressor micro RNA miR-145 and onco micro RNAs miR-21 and miR-222 expressions are differentially modulated by Hepatitis B virus X protein in malignant hepatocytes

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-14-721

    HBx modulated expressions of target proteins in transiently transfected and HBV producing malignant hepatocytes. (A) Western blot confirmed proteins PTEN, p27 and MAP3K (Raf 1) – targets of miR-21, miR-222 and miR-145 respectively were increased accordingly in HBx transfected HepG2 cells. Cells were transfected with 1 μg and 2 μg of HBx plasmid respectively or pCXN2 as a control plasmid. (B) Expressions of PTEN, p27 and MAP3K (Raf 1) proteins in 1.3 fold HBV transfected HepG2 cells. HepG2 cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 vector as a control. (C) Western blot exhibited differential expression of target proteins PTEN, p27 and MAP3K (Raf 1) in constitutively HBV producing HepG2.2.15 and control HepG2 cell line. Cells were collected for protein analysis 48 h after each transfection. β actin was taken as endogenous control. Protein bands were quantified using dentiometric scanner (Bio-Rad). Below are the graphical representation of intensity ratio between target proteins (PTEN, p27 and MAP3K (Raf 1)) and β actin in each lane.
    Figure Legend Snippet: HBx modulated expressions of target proteins in transiently transfected and HBV producing malignant hepatocytes. (A) Western blot confirmed proteins PTEN, p27 and MAP3K (Raf 1) – targets of miR-21, miR-222 and miR-145 respectively were increased accordingly in HBx transfected HepG2 cells. Cells were transfected with 1 μg and 2 μg of HBx plasmid respectively or pCXN2 as a control plasmid. (B) Expressions of PTEN, p27 and MAP3K (Raf 1) proteins in 1.3 fold HBV transfected HepG2 cells. HepG2 cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 vector as a control. (C) Western blot exhibited differential expression of target proteins PTEN, p27 and MAP3K (Raf 1) in constitutively HBV producing HepG2.2.15 and control HepG2 cell line. Cells were collected for protein analysis 48 h after each transfection. β actin was taken as endogenous control. Protein bands were quantified using dentiometric scanner (Bio-Rad). Below are the graphical representation of intensity ratio between target proteins (PTEN, p27 and MAP3K (Raf 1)) and β actin in each lane.

    Techniques Used: Transfection, Western Blot, Plasmid Preparation, Expressing



    Similar Products

    dentiometric scanner  (Bio-Rad)
    90
    Bio-Rad dentiometric scanner
    HBx modulated expressions of target proteins in transiently transfected and HBV producing malignant hepatocytes. (A) Western blot confirmed proteins PTEN, p27 and MAP3K (Raf 1) – targets of miR-21, miR-222 and miR-145 respectively were increased accordingly in HBx transfected HepG2 cells. Cells were transfected with 1 μg and 2 μg of HBx plasmid respectively or pCXN2 as a control plasmid. (B) Expressions of PTEN, p27 and MAP3K (Raf 1) proteins in 1.3 fold HBV transfected HepG2 cells. HepG2 cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 vector as a control. (C) Western blot exhibited differential expression of target proteins PTEN, p27 and MAP3K (Raf 1) in constitutively HBV producing HepG2.2.15 and control HepG2 cell line. Cells were collected for protein analysis 48 h after each transfection. β actin was taken as endogenous control. Protein bands were quantified using <t>dentiometric</t> scanner (Bio-Rad). Below are the graphical representation of intensity ratio between target proteins (PTEN, p27 and MAP3K (Raf 1)) and β actin in each lane.
    Dentiometric Scanner, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dentiometric scanner/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    dentiometric scanner - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    HBx modulated expressions of target proteins in transiently transfected and HBV producing malignant hepatocytes. (A) Western blot confirmed proteins PTEN, p27 and MAP3K (Raf 1) – targets of miR-21, miR-222 and miR-145 respectively were increased accordingly in HBx transfected HepG2 cells. Cells were transfected with 1 μg and 2 μg of HBx plasmid respectively or pCXN2 as a control plasmid. (B) Expressions of PTEN, p27 and MAP3K (Raf 1) proteins in 1.3 fold HBV transfected HepG2 cells. HepG2 cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 vector as a control. (C) Western blot exhibited differential expression of target proteins PTEN, p27 and MAP3K (Raf 1) in constitutively HBV producing HepG2.2.15 and control HepG2 cell line. Cells were collected for protein analysis 48 h after each transfection. β actin was taken as endogenous control. Protein bands were quantified using dentiometric scanner (Bio-Rad). Below are the graphical representation of intensity ratio between target proteins (PTEN, p27 and MAP3K (Raf 1)) and β actin in each lane.

    Journal: BMC Cancer

    Article Title: Tumor suppressor micro RNA miR-145 and onco micro RNAs miR-21 and miR-222 expressions are differentially modulated by Hepatitis B virus X protein in malignant hepatocytes

    doi: 10.1186/1471-2407-14-721

    Figure Lengend Snippet: HBx modulated expressions of target proteins in transiently transfected and HBV producing malignant hepatocytes. (A) Western blot confirmed proteins PTEN, p27 and MAP3K (Raf 1) – targets of miR-21, miR-222 and miR-145 respectively were increased accordingly in HBx transfected HepG2 cells. Cells were transfected with 1 μg and 2 μg of HBx plasmid respectively or pCXN2 as a control plasmid. (B) Expressions of PTEN, p27 and MAP3K (Raf 1) proteins in 1.3 fold HBV transfected HepG2 cells. HepG2 cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 vector as a control. (C) Western blot exhibited differential expression of target proteins PTEN, p27 and MAP3K (Raf 1) in constitutively HBV producing HepG2.2.15 and control HepG2 cell line. Cells were collected for protein analysis 48 h after each transfection. β actin was taken as endogenous control. Protein bands were quantified using dentiometric scanner (Bio-Rad). Below are the graphical representation of intensity ratio between target proteins (PTEN, p27 and MAP3K (Raf 1)) and β actin in each lane.

    Article Snippet: Proteins bands were quantified using Dentiometric scanner (Bio-Rad-GS-800, USA).

    Techniques: Transfection, Western Blot, Plasmid Preparation, Expressing